This manual aims to help trainers enhance their understanding of the gender dimensions of the HIV/AIDS epidemic. The manual is divided into four sections. Section 1 highlights the basic facts on HIV/AIDS and the growing global, national and regional challenges. Section 2 outlines a one-day training module on “Gender Concerns in HIV/AIDS in Development” and includes suggestions for structure, agenda, methodology and training aids for the facilitator. Section 3 outlines a two-day training module on “Gender Concerns in HIV/AIDS: A Human Rights Approach”. The fourth and last section pulls together the lessons learned during the use of the modules, both from the perspective of the facilitator and the participants.